The Autosampler holds a removable 68-vial rotating sample rack. Firstly, ensure make sure that the main power switch is on the 'on' position. Silanisation of syringe - TOC determination of surfactant 5.13. Detailed calibration curve information can easily be referenced when setting the measurement conditions. The pressure of the carrier gas i.e. When creating the calibration curve, the optimal measurement conditions are displayed when the standard solution concentration is set. On the TOC Control screen toolbar, select View > Calibration Curve List.. 3. 6.1 Shimadzu TOC-VCSH Analyzer. Standard TOC Solutions. This page may contain references to products that are not available in your country. If HCl is low, replace with 2N HCl located in media bottle in acid cabinet 5.2 Operate the instrument as per S.O.P. TOC-L - Preparing your instrument for Startup . Figure 1 Filling up the . TOC - measurement principle - catalytic combustion at 680°C 5.10. 1. Calibration curve :5-point calibration curve generated using TOC 0 - 0.1 - 0.5 - 1.0 - 2.0 mgC/L potassium hydrogen . If analysis of total nitrogen is also desired, you should select the TOC-V as it is coupled with the TN module. Calibrate the instrument for power spectrum, resolution, wave number accuracy, repeatability of wave-number and repeatability of absorbance. IC : Dissolve 0.875 gm NaHCO3 and 1.1025 gm of Na2CO3 in 500 ml of water to get 500 ppm IC standard and . Initialized the instrument. Otherwise, the selection of an analyzer is largely On the TOC Control screen toolbar, select View > Calibration Curve List.. 3. Total Organic Carbon Analysis TOC and TN Measurements of Seawater LAAN-A-TC-E038 For pollution control of the ocean and in the research of the ocean environment, the measurement of total organic carbon (TOC) is regarded as an important index of organic contaminants contained in seawater. 5.3 Performance check of TOC Create an entry in the Shimadzu log book. Make sure to enter calibration curve info when run is complete. TOC determination with wet chemical UV-oxidation 5.12. 6.1.1 Shimadzu ASI-V Autosampler . 5.7. The TOC Carrier Gas Pressure Regulator should read 200KPa ALLOW 60 MINUTES OF WARM-UP TIME TOC Calibration Table Setup 1. 1.1. 6.4 Laboratory glassware and pipettes . System Preparation The following System Preparation step are valid for all TOC-L instruments. Note the following: start date, start time, # of samples (not including STD?s and BLK?s), operator, and the name of the project. Calibration with automatic dilution function 5.8. Click in the first line of the Sample Table and select Edit > Insert Standard.. 2. If analysis of total nitrogen is also desired, you should select the TOC-V as it is coupled with the TN module. At the same time, the measurement of total nitrogen (TN), This procedure is applicable to calibrate Total Organic Carbon Analyzer. Procedure. One vial of blank water and one vial each of 500, 1000, and 2000 µg/L sucrose for TOC calibration and validation. Blank value consideration in TOC analysis 5.9. Not for use in diagnostic procedures. 3.0 Responsibility 3.1 Doing: Technical Assistant 3.2 Checking: Executive /Manager 4.0 Accountability Head of the Department 5.0 Procedure 5.1 Clean the instrument with a dry cotton cloth. TOC-V WP Features. 7.0 PROCEDURE CALIBRATION OF TOTAL ORGANIC CARBON (TOC) ANALYZER: Four Point Calibration of Total Organic Carbon (TOC) Analyzer: Set the MQ water as zero ppb and prepare 250ppb, 500 ppb and 1000ppb solutions of KHP in MQ water. The modular design of Shimadzu's TOC series supports straightforward combination with a solid sample module. The SSM-5000A module can be used in combination with TOC-L a. System Preparation The following System Preparation step are valid for all TOC-L instruments. 6.1.3 Data Collection Software . Zero air should be 2 Kg/cm 2 (200 kpa) and flow should be 150 ml/min. 6.1.5 CO. 2 Absorber (soda lime) 6.2 "Ultra Zero Air" compressed air and regulator . Continuous TOC determination in the chemical industry 6.4. 1.6 This procedure conforms to EPA Method 415.1. 7.0 PROCEDURE CALIBRATION OF TOTAL ORGANIC CARBON (TOC) ANALYZER: Four Point Calibration of Total Organic Carbon (TOC) Analyzer: Set the MQ water as zero ppb and prepare 250ppb, 500 ppb and 1000ppb solutions of KHP in MQ water. 6.3. Concerned Department Heads shall be responsible for . Standard TOC Solutions. the procedure of Table 1, using the Shimadzu TOC-L CPH combustion type TOC analyzer. 5.2 Operate the instrument as per S.O.P. Zero air should be 2 Kg/cm 2 (200 kpa) and flow should be 150 ml/min. TC : Dissolve 0.5950 gm. From the TOC Control screen select File > Open Sample Table and open a new or previously saved calibration Sample Table (Fig 8).. 1. The Autosampler is designed to operate on samples or standards prepared in septa sealed 40 ml glass vials. 6.1.3 Data Collection Software . Solution Set-Up. Total Organic Carbon Analysis TOC System Suitability Test for Sterile Water . 8. 6.1 Shimadzu TOC-VCSH Analyzer. Performance check of TOC. IC : Dissolve 0.875 gm NaHCO3 and 1.1025 gm of Na2CO3 in 500 ml of water to get 500 ppm IC standard and . For this purpose, a solid-sample module (SSM-5000A) was connected to the main instrument, either a TOC-L series combustion system or the wet-chemical model of the TOC-V series. Ensure that all connections of the instrument are proper. 638-60257-91. of sucrose in 500 ml of water to get 500 ppm of carbon and further dilute with water to get final concentration 250 ppb, 500 ppb and 750 ppb of carbon. From the TOC Control screen select File > Open Sample Table and open a new or previously saved calibration Sample Table (Fig 8).. 1. 2. Continuous TOC determination on airports 6.8 TOC-4200 - Measurement range up to 55,000mg/L TOC Open Carrier Gas used for TOC (Zero air Cylinder) with Cylinder pressure maintained and Gas line pressure maintained at 4-6 Kg/cm 2.Power "ON" the instrument. 1. Action. Moreover, the ability to auto-generate a calibration curve from a single standard, coupled with the SCA-130-513. 1.0 OBJECTIVE:To lay down a Standard Operating Procedure for Operation and Calibration of Total Organic Carbon (TOC) Analyzer. of sucrose in 500 ml of water to get 500 ppm of carbon and further dilute with water to get final concentration 250 ppb, 500 ppb and 750 ppb of carbon. Set of -500-1000-2000 µg/L Sucrose. 1.1. Startup the Shimadzu TOC Analyzer by creating a sample table and connecting to the instrument via the software: a. Click on the TOC Control V icon b. The instrument was . Instrument will begin to run the startup procedure 6. Open Carrier Gas used for TOC (Zero air Cylinder) with Cylinder pressure maintained and Gas line pressure maintained at 4-6 Kg/cm 2.Power "ON" the instrument. Silanisation of syringe - TOC determination of surfactant 5.13. calibration, drifting base lines), they may be certified as a technician. Calibration of TOC Analyzer Preparation of the working standard solutions for the calibration curve Prepare organic and inorganic carbon calibration series and/or total nitrogen and nitrate/nitrite calibration series by diluting the stock solutions to cover the expected range in the samples. Note the following: start date, start time, # of samples (not including STD?s and BLK?s), operator, and the name of the project. The Shimadzu TOC-L total organic carbon analyzer is ideal for TOC measurement by Standard Methods 5310B. 6.1.2 Instrument Controller . The individual steps for the PC-controlled or Standalone instruments are explained in Chapter 2 and 3. Continuous condensate monitoring using the TOC-4200 6.5. Otherwise, the selection of an analyzer is largely Do the 4 point calibration by selecting dilution from standard solution. One vial of blank water and one vial each of 500, 1000, and 2000 µg/L sucrose for TOC calibration and validation. TOC determination with wet chemical UV-oxidation 5.12. 1. Set of 0-2000 µg/L Sucrose Standard TOC. TOC Determination with Solid Module SSM-5000A No. Please contact us to check the availability of these products in your country. 3.0 Responsibility 3.1 Doing: Technical Assistant 3.2 Checking: Executive /Manager 4.0 Accountability Head of the Department 5.0 Procedure 5.1 Clean the instrument with a dry cotton cloth. Step. TOC - measurement principle - catalytic combustion at 680°C 5.10. Create an entry in the Shimadzu log book. Blank value consideration in TOC analysis 5.9. 1. 6.3 40 mL borosilicate vials and septum caps . Both use high-temperature combustion with the same proprietary catalyst and NDIR detection. 2.0 SCOPE:This procedure shall apply for Operation and Calibration of Total Organic Carbon (TOC) Analyzer..3.0 RESPONSIBILITY: All concerned personnel shall responsible to follow the procedure mention in this SOP. 6.1.2 Instrument Controller . Ensure that all connections of the instrument are proper. Operate the instrument as per S.O.P. Set of -500-1000-2000 µg/L Sucrose. When creating the calibration curve, the optimal measurement conditions are displayed when the standard solution concentration is set. 5.3 Performance check of TOC TOC-4200 with high sensitivity option 6.6. On the Calibration Curve List dialog box, select New (Fig 9).. 4 . 6.1.1 Shimadzu ASI-V Autosampler . Total Organic Carbon Analysis TOC System Suitability Test for Sterile Water . User Guide 3.1 Instrument Start-Up 1. 6.1.4 High Sensitivity Catalyst . Relevant Instruction Manuals Name Content TOC-V User Manual Describes the procedures necessary for performing routine operation and maintenance of the TOC-V. TOC-V Administrator's Manual Describes the procedures necessary for safe administration of the TOC-Control V software, Adjust them if necessary by opening the front door and . TOC-L - detection limit normal catalyst 5.11. 9.2 Halogen scrubber (Shimadzu PN 630-00992) 9.3 Sulfate scrubber (Shimadzu PN 220-95268-01) 9.4 Membrane filter (Shimadzu PN 046-00042-12) 6.1.5 CO. 2 Absorber (soda lime) 6.2 "Ultra Zero Air" compressed air and regulator . SUMMARY 2.1 The Shimadzu TOC-L uses a high temperature combustion method to analyze aqueous samples for total carbon (TC), total organic carbon (TOC) and dissolved organic carbon (DOC), also known as non-purge-able organic carbon (NPOC). batch TOC analyzers with autosamplers: the Shimadzu TOC-5000A, and the Shimadzu TOC-V. Clean the humidifier bottle and fill it up with pure water. Calibration with automatic dilution function 5.8. Click on the icon Measurement, select "EP5.0 validation". Enter appropriate information on 'TOC' form, and then close form to save data. In most cases this switch is left on. 2. Comprehensive security functions such as password input and operator access restrictions. Clean the humidifier bottle and fill it up with pure water. Adjust them if necessary by opening the front door and . On the Calibration Curve List dialog box, select New (Fig 9).. 4 . contact your Shimadzu representative. Startup the Shimadzu TOC Analyzer by creating a sample table and connecting to the instrument via the software: a. Click on the TOC Control V icon b. batch TOC analyzers with autosamplers: the Shimadzu TOC-5000A, and the Shimadzu TOC-V. Click in the first line of the Sample Table and select Edit > Insert Standard.. 2. Procedure. This procedure is applicable to calibrate Total Organic Carbon Analyzer. 6.1.4 High Sensitivity Catalyst . Set of 0-2000 µg/L Sucrose Standard TOC. The TOC-L, with a 680 °C furnace and platinum catalyst, utilizes high-temperature catalytic oxidation to completely oxidize organic carbon. - Automatic setting of optimal measurement conditions. 6.3 40 mL borosilicate vials and septum caps . Step. TOC-V WP Features. 6.4 Laboratory glassware and pipettes . 5.7. This switch is located at the upper right corner of the right side of the instrument next to the computer screen. Calibration curve :5-point calibration curve generated using TOC 0 - 0.1 - 0.5 - 1.0 - 2.0 mgC/L potassium hydrogen . The pressure of the carrier gas i.e. The TOC software is able to monitor various calibration curves (MAXIMUM of THREE) to apply . Wizards allow simple input of measurement and calibration settings. Detailed calibration curve information can easily be referenced when setting the measurement conditions. 638-60257-91. Reagants. 9 Equipment and supplies 9.1 Shimadzu TOC-VCPH analyzer with TNM-1 and ASI-V autosampler. The HCl and Phosphoric acid (usually replaced w/ DI) bottles should be at least a quarter full. Shimadzu TOC-V CPH/CPN (NPOC/TC/TN Modes) Introduction The Shimadzu TOC-V . Both use high-temperature combustion with the same proprietary catalyst and NDIR detection. For TC determination, the swab is placed . Operate the instrument as per S.O.P. The individual steps for the PC-controlled or Standalone instruments are explained in Chapter 2 and 3. One vial of blank water and one vial of 500 µg/L sucrose for TOC calibration. - Automatic setting of optimal measurement conditions. Action. Figure 1 Filling up the . TOC-L - Preparing your instrument for Startup . Moreover, the ability to auto-generate a calibration curve from a single standard, coupled with the Ensure that the Instrument is ready for the calibration and start-up the procedure is followed. TOC-L - detection limit normal catalyst 5.11. Do the 4 point calibration by selecting dilution from standard solution. The Shimadzu TOC-L total organic carbon analyzer is ideal for TOC measurement by Standard Methods 5310B. 6.5 . After the first injection the 8. TC : Dissolve 0.5950 gm. Performance check of TOC. function is shown in the following calibration curve: Shimadzu Europa GmbH www.shimadzu.de Albert-Hahn-Str.6-10, 47269 Duisburg Stock solution Calibration Curve 500mg/L Measurement of an unknown sample In this case a high concentrated sample (over 6000mg/L) is measured against a 500mg/L calibration curve. In this way, the instruments can also be employed for the analysis of solids. The TOC-L, with a 680 °C furnace and platinum catalyst, utilizes high-temperature catalytic oxidation to completely oxidize organic carbon. TOC Analyzers The Utility of Shimadzu TOC Analyzers Features Flow Line Diagrams PC-Controlled Models Standalone Models Space-Saving and Energy-Saving Design, Other Functions and Features ASI-L Autosampler and OCT-L 8-Port Sampler TNM-L Total Nitrogen Unit and SSM-5000A Solid Sample Combustion Unit Compatibility of Options and Special Accessories the procedure of Table 1, using the Shimadzu TOC-L CPH combustion type TOC analyzer. Shimadzu TOC-L Analyzer Manual Revision 1.1 (19-10-2018) 2 3. The instrument was . Total TOC analyzer operation, control, and data collection are handled by personal computer running enhanced software. 6.5 . One vial of blank water and one vial of 500 µg/L sucrose for TOC calibration. The modular design of Shimadzu's TOC-L series now enables the additional determination of the swabs using the same instrument. The instrumentation for DOC/TOC analysis consists of two interconnected modules: the Shimadzu TOC-V CSH Analyzer, and the Shimadzu ASI-V Autosampler. The figure below is a schematic diagram showing TOC measurement using the 680°C combustion catalytic oxidation method and the NDIR method.